Species | Human | ||||
Protein Construction |
|
||||
Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
||||
Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human VEGF165, No Tag at 5μg/ml (100μl/well) on the plate can bind Neuropilin-1, His, Human. Test result was comparable to standard batch. | ||||
Expression System | HEK293 | ||||
Theoretical Molecular Weight | 70.97 kDa | ||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. | ||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Target Background | Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta. |
Synonyms | BDCA4; NP1; Neuropilin-1; NRP1; CD304; VEGF165R; Neuropilin 1; NRP; Transmembrane Receptor; CD304 Antigen |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.