| Species |
Human |
| Protein Construction |
| His |
Latent TGF beta 2/TGFB2 (Leu21-Ser414)_x000D_
Accession # P61812-1 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Human TGFbeta RII, hFc Tag captured on CM5 Chip via Protein A can bind Latent TGF beta 2/TGFB2, His, Human in SPR assay (Biacore T200). Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
46.6 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 40-60 kDa&12-13 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs. |
| Synonyms |
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
