| Species |
Human |
| Protein Construction |
KLKB1 (Gly20-Ala638)_x000D_
Accession # P03952 |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its ability to cleave a flourogenic peptide substrate ProPheArg7amido4methylcoumarin (PFRAMC). The specific activity is > 1000 pmol/min/μg. Test result meets the standard. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
70.3 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 72-80 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 20mM NaAc,150mM NaCl (pH 5.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc,150mM NaCl (pH 5.0). |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides. |
| Synonyms |
Plasma kallikrein; PKK; KLK3; KLKB1; Fletcher factor; Kininogenin |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
