| ￥3500 | |
| Z05515 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | IL-7R alpha/CD127, His, Human captured on CM5 Chip via antihis antibody can bind Human IL7, No Tag in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 25.7 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of IL-7R alpha/CD127, His, Human is greater than 95% as determined by SEC-HPLC. »
IL-7R alpha/CD127, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-7R alpha/CD127, His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-7, No Tag with an affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). »
| Target Background | Interleukin 7 (IL-7) and its receptor (IL-7R, a heterodimer of IL-7Rα and γc) are essential for normal lymphoid development. IL-7 and IL-7R activate three main pathways: STAT5, PI3K/Akt/mTOR and MEK/Erk, ultimately leading to the promotion of leukemia cell viability, cell cycle progression and growth. |
| Synonyms | IL-7R alpha; IL7Ra; CD127; CDW127; IL7Rα |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
