| ￥2300 | |
| Z05512-100 | |
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| Species | Human | |
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 0.01EU per μg by the LAL method. | |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized IL-7, Human at 2μg/ml (100μl/Well) on the plate can bind Human IL7R alpha, hFc Tag. Test result was comparable to standard batch. Measured in a cell proliferation assay using murine 2E8 cells. The ED50 for this effect is 0.1 0.5 ng/mL. Test result was comparable to standard batch. |
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| Expression System | E.coli | |
| Theoretical Molecular Weight | 17.5 kDa | |
| Apparent Molecular Weight | The protein has a predicted MW of 17.5 kDa same as Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 8.0). | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | |
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background | Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor. |
| Synonyms | IL7; Interleukin-7; IL-7; Lymphopoietin-1; PBGF |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
