| ￥3500 | |
| Z05510-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 65.3 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded IL-6 R alpha/CD126 hFc Chimera, Human, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime). »
Immobilized Human IL-6, No Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve forIL-6 R alpha/CD126 hFc Chimera, Human, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. »
Immobilized IL-6 R alpha/CD126 hFc Chimera, Human, hFc Tag at 2 μg/ml(100 μl/well) on the Human IL-6, No Tag precoated plate(5 μg/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA. »
The purity of IL-6 R alpha/CD126 hFc Chimera, Human is greater than 90% as determined by SEC-HPLC. »
IL-6 R alpha/CD126 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Serum levels of interleukin-6 (IL-6) are increased in patients with type 2 diabetes (T2D). IL-6 exerts its pleiotropic effects via the IL-6 α-receptor (IL-6R), which exists in membrane-bound and soluble (sIL-6R) forms and activates cells via the β-receptor glycoprotein 130 (gp130). |
| Synonyms | IL-6R subunit alpha; IL-6R-alpha; IL-6RA; IL-6R 1; IL6R; CD126; IL6RQ; gp80 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
