| ￥3000 | |
| Z05489-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized IL-27RA/TCCR hFc Chimera, Human at 5μg/ml (100μl/well) on the plate can bind Human IL27, His Tag. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 79.3 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS, 5mM DTT (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized IL-27RA/TCCR hFc Chimera, Human, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9 ng/ml determined by ELISA. »
IL-27RA/TCCR hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-27RA/TCCR hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). »
| Target Background | A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects. |
| Synonyms | IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
