Species | Human | ||||
Protein Construction |
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Purity | > 95% as determined by Bis-Tris PAGE | ||||
Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human IL27 RA, hFc Tag at 5μg/ml (100μl/well) on the plate can bind IL-27, His, Human. Test result was comparable to standard batch. | ||||
Expression System | CHO | ||||
Theoretical Molecular Weight | 50.6 kDa | ||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 54-64 kDa based on Bis-Tris PAGE result. | ||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). | ||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human IL-27 RA, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-27, His, Human, His Tag with the EC50 of 37.7 ng/ml determined by ELISA. »
IL-27, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind IL-27, His, Human, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). »
Target Background | Interleukin (IL)-27, a member of the IL-6/IL-12 family, has an important role in modulating inflammation in partnership with innate and adaptive immune cells. |
Synonyms | Interleukin-27; p28; IL30; IL-27; IL-27A; IL27; p28; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.