目录产品 » IL-2 R beta&IL-2 R gamma hFc Chimera, Human
Manual
SDS
COAs

IL-2 R beta&IL-2 R gamma hFc Chimera, Human

Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change
￥3500
Z05471

产品简介
Species Human
Protein Construction
IL-2 R beta & IL-2 R gamma [Ala27-Thr240 (IL-2 R beta) & Leu23-Ala262 (IL-2 R gamma)]
Accession # P14784 (IL-2 R beta) & P31785-1 (IL-2 R gamma)		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human IL­15RA&IL­15, His Tag at 2μg/ml (100μl/well) on the plate can bind IL-2 R beta&IL-2 R gamma hFc Chimera, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
返回

技术背景
Target Background Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change
Synonyms IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ
返回

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.