目录产品 » IL-2 R beta&IL-2 R gamma hFc Chimera, Human
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IL-2 R beta&IL-2 R gamma hFc Chimera, Human

Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change
￥3500
Z05471-100

产品简介
Species Human
Protein Construction
IL-2 R beta & IL-2 R gamma [Ala27-Thr240 (IL-2 R beta) & Leu23-Ala262 (IL-2 R gamma)]
Accession # P14784 (IL-2 R beta) & P31785-1 (IL-2 R gamma)		 hFc
N-term C-term
Purity > 90% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
IL-2 R Beta&IL-2 R Gamma HFc Chimera, Human

Immobilized Human IL-15Ra&IL-15, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag with the EC50 of 14.5ng/ml determined by ELISA. »

IL-2 R Beta&IL-2 R Gamma HFc Chimera, Human

IL-2 R beta&IL-2 R gamma hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. »

IL-2 R Beta&IL-2 R Gamma HFc Chimera, Human

IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). »

IL-2 R Beta&IL-2 R Gamma HFc Chimera, Human

IL-2 R beta&IL-2 R gamma hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change
Synonyms IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma; IL-2R subunit β; IL-2 R β&IL-2 R gamma; IL-2Rγ; IL-2R γ; IL-2 Rγ; IL-2 R γ
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.