| ￥2500 | |
| Z05463-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human IL1 Beta, His Tag at 5μg/ml (100μl/Well) on the plate can bind IL-1R2/IL-1 RII/CD121b hFc Chimera, Human. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 64.4 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human IL-1 Beta, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-1R2/IL-1 RII/CD121b hFc Chimera, Human, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA. »
IL-1R2/IL-1 RII/CD121b hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-1R2/IL-1 RII/CD121b hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). »
| Target Background | The IL-1 type II receptor (decoy RII) is a nonsignaling molecule the only established function of which is to capture IL-1 and prevent it from interacting with signaling receptor. The decoy RII is released in a regulated way from the cell surface. |
| Synonyms | IL-1R-2; IL-1RT-2; IL-1RT2; CDw121b; IL-1R-beta; CD121b; IL1RB; IL1R2; IL-1 RII; MGC47725; IL-1R-β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
