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IL-1R1, His, Human

Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
￥3000
Z05461-100

产品简介
Species Human
Protein Construction
IL-1R1 (Leu18-Thr332)_x000D_
Accession # NP_000868.1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 37 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
IL-1R1, His, Human

Immobilized IL-1R1, His, Human, His Tag at 5 μg /ml(100 μl/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Avi Tag with the EC50 of 58.8ng/ml determined by ELISA. »

IL-1R1, His, Human

The purity of IL-1R1, His, Human is greater than 95% as determined by SEC-HPLC. »

IL-1R1, His, Human

IL-1R1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

IL-1R1, His, Human

IL-1R1, His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
Synonyms IL-1R-1; IL-1RT1; IL-1R-alpha; CD121a; IL-1 RI; IL1R; IL1RI; IL-1RI; p80; D2S1473; IL1RA
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.