| ￥1500 | |
| Z05453-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human IL18 at 5μg/ml (100μl/Well) on the plate can bind IL-18BP hFc Chimera, Human. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 44.4 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human IL-18, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for IL-18BP hFc Chimera, Human, hFc Tag with the EC50 of 16.9 ng/ml determined by ELISA. »
IL-18BP hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
IL-18BP hFc Chimera, Human, hFc Tag captured on Protein A chip, can bind Human IL-18 with an affinity constant of 0.04nM as determined in a SPR assay (Biacore T200). »
The purity of IL-18BP hFc Chimera, Human is greater than 90% as determined by SEC-HPLC. »
| Target Background | Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice. |
| Synonyms | IL-18BP; Igifbp |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
