IL-17A/CTLA-8, His, Human

Interleukin‑17A (IL‑17A), also known as CTLA‑8, is a 15‑20 kDa glycosylated cytokine that plays an important role in anti‑microbial and chronic inflammation. The six IL‑17 cytokines (IL‑17A‑F) are encoded by separate genes but adopt a conserved cystine knot fold.IL-17A is a ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.

价格	￥3500
产品编号	Z05444-100
规格	100 μg 500 μg 大单询价
数量
TAT

产品简介图例技术背景

产品简介

Species

Human

Protein Construction

IL-17A/CTLA-8 (Gly24-Ala155) Accession # Q16552-1	His
N-term	C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

16.2 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 15-20 kDa and 25-30 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

图例

Immobilized IL-17A/CTLA-8, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-17R alpha, hFc Tag with the EC50 of 72.2ng/ml determined by ELISA. »

Immobilized IL-17A/CTLA-8, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-IL-17A Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA. »

Immobilized IL-17A/CTLA-8, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-17Rc, hFc Tag with the EC50 of 29.8ng/ml determined by ELISA. »

The purity of IL-17A/CTLA-8, His, Human is greater than 95% as determined by SEC-HPLC. »

IL-17A/CTLA-8, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Human IL-17R alpha, His Tag immobilized on CM5 Chip can bind IL-17A/CTLA-8, His, Human, His Tag with an affinity constant of 1.57nM as determined in SPR assay (Biacore T200) (Routine Test). »

技术背景

Target Background

Synonyms

CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.