| ￥2000 | |
| Z05427-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 20.24 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in 20 mM Citric acid (pH 3.0). | ||||
| Reconstitution | Dissolve the lyophilized protein in 20 mM Citric acid (pH 3.0).It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/ml for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 1-8 ng/mL. (QC Test) »
Immobilized IL-11, His, Human, His Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. »
The purity of IL-11, His, Human is greater than 95% as determined by SEC-HPLC. »
IL-11, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind IL-11, His, Human, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). »
| Target Background | Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers, human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions. |
| Synonyms | Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
