| ￥3000 | |
| Z05417-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Human IL1R1, His Tag captured on CM5 Chip via AntiHis Antibody can bind IL-1 alpha/IL-1A hFc Chimera, Human in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 43.99 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
The purity of IL-1 alpha/IL-1A hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
IL-1 alpha/IL-1A hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human IL-1R1, His Tag captured on CM5 Chip via Anti-His Antibody can bind IL-1 alpha/IL-1A hFc Chimera, Human, hFc Tag with an affinity constant of 0.28 μM as determined in SPR assay (Biacore T200). »
| Target Background | The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases. |
| Synonyms | Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
