HGF R/c-MET hFc Chimera, Human
¥2100 | |
Z05376-100 | |
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¥2100 | |
Z05376-100 | |
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Species | Human | ||||
Protein Construction |
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Purity | > 95% as determined by Bis-Tris PAGE | ||||
Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human HGF, His Tag at 2μg/ml (100μl/Well) on the plate can bind HGF R/c-MET hFc Chimera, Human. Test result was comparable to standard batch. | ||||
Expression System | HEK293 | ||||
Theoretical Molecular Weight | 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera) | ||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result. | ||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Human HGF, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for HGF R/c-MET hFc Chimera, Human, hFc Tag with the EC50 of 7.0 ng/ml determined by ELISA. »
HGF R/c-MET hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Immobilized HGF R/c-MET hFc Chimera, Human, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-HGF R Antibody, hFc Tag with the EC50 of 4.2 ng/ml determined by ELISA. »
The purity of HGF R/c-MET hFc Chimera, Human is greater than 90% as determined by SEC-HPLC. »
Target Background | c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling. |
Synonyms | MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.