| ￥3000 | |
| Z05362-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 35.3 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Human GM-CSF R alpha, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-GM-CSF R alpha Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. »
Immobilized Human GM-CSF at 5 μg/ml(100 μl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, His Tag with the EC50 of 1.0 μg/ml determined by ELISA. »
The purity of Human GM-CSF R alpha is greater than 95% as determined by SEC-HPLC. »
Human GM-CSF R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human GM-CSF R alpha , His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200). »
| Target Background | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
| Synonyms | GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
