GM-CSF, Human
| ¥2500 | |
| Z05361-100 | |
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| ¥2500 | |
| Z05361-100 | |
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| Species | Human | |
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | |
| Endotoxin Level | Less than 0.1EU per μg by the LAL method. | |
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | |
| Expression System | E.coli | |
| Theoretical Molecular Weight | 14.6 kDa | |
| Apparent Molecular Weight | The protein has a predicted MW of 14.6 kDa same as Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | |
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test). »
Immobilized GM-CSF, Human, No Tag at 2 μg/ml(100 μI/well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. »
The purity of GM-CSF, Human is greater than 95% as determined by SEC-HPLC. »
GM-CSF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind GM-CSF, Human, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200). »
| Target Background | Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. |
| Synonyms | CSF; CSF2; GMCSF; GM-CSF; MGC131935; MGC138897 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.