| Species |
Human |
| Protein Construction |
GDNF (Ser78-Ile211)
Accession # P39905-1 |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 0.1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized GDNF, Human at 1 μg/ml (100μl/well) on the plate can bind Human GFR alpha 1, His Tag. Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
15.06 kDa |
| Apparent Molecular Weight |
The protein has a predicted MW of 15.06 kDa same as Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
Glial cell line-derived neurotrophic factor (GDNF) is a 134 amino acid protein belonging in the GDNF family ligands (GFLs). GDNF was originally isolated from rat glial cell lines and identified as a neurotrophic factor with the ability to promote dopamine uptake within midbrain dopaminergic neurons. |
| Synonyms |
Glial cell line-derived neurotrophic factor; hGDNF; Astrocyte-derived trophic factor (ATF) |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
