Immobilized GDF15, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 14.0 ng/ml determined by ELISA.

GDF15, His, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind GDF15, His, Human, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

GDF15, His, Human

Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.

产品编号	Z05341
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


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产品简介

Species

Human

Protein Construction

His	GDF15 (Ala197-Ile308) Accession # Q99988-1
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized GDF15, His, Human at 0.5μg/ml (100μl/well) on the plate can bind Human GFRAL, hFc Tag. Test result was comparable to standard batch.

Expression System

E.coli

Theoretical Molecular Weight

13.5 kDa

Apparent Molecular Weight

The protein migrates to 15-16 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9).

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Immobilized GDF15, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 14.0 ng/ml determined by ELISA.

GDF15, His, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind GDF15, His, Human, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200).

技术背景

Target Background	Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Synonyms	GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.