| ￥3500 | |
| Z05341-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized GDF15, His, Human at 0.5μg/ml (100μl/well) on the plate can bind Human GFRAL, hFc Tag. Test result was comparable to standard batch. | ||||
| Expression System | E.coli | ||||
| Theoretical Molecular Weight | 13.5 kDa | ||||
| Apparent Molecular Weight | The protein migrates to 15-16 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). | ||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized GDF15, His, Human, His Tag at 0.5 μg/ml (100 μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 14.0 ng/ml determined by ELISA. »
GDF15, His, Human on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%. »
Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind GDF15, His, Human, His Tag with an affinity constant of 0.014 nM as determined in SPR assay (Biacore T200). »
Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : BiotinylatedGDF15, His, Human, His Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 40.8ng/ml. »
| Target Background | Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
| Synonyms | GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
