| Species |
Human |
| Protein Construction |
GARP (G139N) & Latent TGF Beta 1 Complex [His20-Leu628 (GARP (G139N)) & Leu30-Ser390 (Latent TGF Beta 1)]
Accession # Q14392 (GARP (G139N)) & P01137(Latent TGF beta 1) |
His |
Avi |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized GARP (G139N) &Latent TGF Beta 1 Complex, His & Avi, Human at 5μg/ml (100μl/well) on the plate can bind AntiGARP&Latent TGF Beta 1 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
70.3 kDa (GARP(G139N))&41.4 kDa (Latent TGF Beta 1) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 75-80 kDa (GARP(G139N))&15 kDa&42-48 kDa (Latent TGF Beta 1) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex. |
| Synonyms |
LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
