| Species |
Human |
| Protein Construction |
| His |
Galectin 3 (Ala2-Ile250)
Accession # P17931 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Galectin 3, His, Human at 1 μg/ml (100μl/Well) on the plate can bind AntiGalectin 3 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
27.1 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 150 mM NaCl, 5 mM EDTA, 2 mM TCEP (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Galectin 3, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC50 of 6.8 ng/ml determined by ELISA.
Galectin 3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain.Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). |
| Synonyms |
AGE-R3; CBP35; GAL3; Gal-3; galactin-3; GALBPCBP35; Galectin3; GALIG; L29; L31; Lectin L-29; LGALS2; LGALS3; Mac-2; MAC2GAL3 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
