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FGF8b, Human

Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.
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Z05294-100

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产品简介
Species Human
Protein Construction
FGF8b (Gln23-Arg215)
Accession # P55075-3
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 0.01EU per μg by the LAL method.
Biological Activity Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. Test result was comparable to standard batch
Expression System E.coli
Theoretical Molecular Weight 22.39 kDa
Apparent Molecular Weight The protein has a predicted MW of 22.39 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20mM Tris, 300mM NaCl (pH 8.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
FGF8b, Human

Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is 5-20 ng/mL. »

FGF8b, Human

FGF8b, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.
Synonyms FGF8b; FGF-8b; FGF-8; FGF8; FGF 8; AIGF; HBGF-8; HBGF8; HBGF 8;
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.