| ￥4100 | |
| Z05291-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 46.9 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized FGF21, mFc Chimera, Avi, Human, mFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 0.75 μg/ml determined by ELISA. »
The purity of FGF21, mFc Chimera, Avi, Human is greater than 95% as determined by SEC-HPLC. »
FGF21, mFc Chimera, Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind FGF21, mFc Chimera, Avi, Human, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200). »
| Target Background | Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia. |
| Synonyms | UNQ3115; PRO10196; FGF-21; FGF21 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
