| ￥2600 | |
| Z05279-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Loaded Fc gamma RIIIB/CD16b (NA1), His, Human on Anti-His-Biosensor can bind Rituximab in BLI assay (Gator® Prime). Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 22.52 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
The purity of Fc gamma RIIIB/CD16b (NA1), His, Human is greater than 95% as determined by SEC-HPLC. »
Fc gamma RIIIB/CD16b (NA1), His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Fc gamma RIIIB/CD16b (NA1), His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 8.55 μM as determined in SPR assay (Biacore T200). »
Loaded Fc gamma RIIIB/CD16b (NA1), His, Human, on Anti-His-Biosensor can bind Rituximab with an affinity constant of 2.27 µM as determined in BLI assay (Gator® Prime). (QC Test) »
| Target Background | Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils. |
| Synonyms | CD16; Fc gamma RIIIB; FCG3; FCGR3B; FcgRIIIB; FCRIIIB; IGFR3; CD16b (NA2); CD16B; FCG3B; FCG3; FCGR3; FCR-10 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
