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EPHB2, His, Human

EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
￥2600
Z05254-100

产品简介
Species Human
Protein Construction
EPHB2 (Val19-Leu543)
Accession # P29323-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 59.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
EPHB2, His, Human

Immobilized EPHB2, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human Ephrin-B2, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA. »

EPHB2, His, Human

Immobilized EPHB2, His, Human at 0.5 μg/ml(100 μl/well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 9.0ng/ml determined by ELISA. »

EPHB2, His, Human

The purity of EPHB2, His, Human is greater than 95% as determined by SEC-HPLC. »

EPHB2, His, Human

EPHB2, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

EPHB2, His, Human

EPHB2, His, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human EPHB2, His Tag with an affinity constant of 82.97 pM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
Synonyms DRT; ERK; HEK5; TYRO5; CAPB; EK5; EPHT3; PCBC; Cek5; EphB2; EPHT3MGC87492; Nuk; Qek2; Sek3
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.