| ￥200 | |
| Z05234-100 | |
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| Species | Human | |
| Protein Construction |
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| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 0.2EU per μg by the LAL method. | |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized EGF, Human at 2μg/ml (100μl/well) on the plate can bind Human EGFR, hFc Tag. Test result was comparable to standard batch. Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 0.2 ng/mL. Test result was comparable to standard batch. |
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| Expression System | E.coli | |
| Theoretical Molecular Weight | 5.6 kDa | |
| Apparent Molecular Weight | The protein has a predicted MW of 5.6 kDa same as Bis-Tris PAGE result. | |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | |
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1 - 0.2 ng/mL (QC Test). »
Immobilized EGF, Human, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.59 μg/ml determined by ELISA. »
The purity of EGF, Human is greater than 95% as determined by SEC-HPLC. »
EGF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF, Human, No Tag with an affinity constant of 1.33 nM as determined in SPR assay (Biacore T200). »
| Target Background | The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family. |
| Synonyms | HOMG4; EGF; URG |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
