| ￥3000 | |
| Z05233-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 32.52 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded EGF hFc Chimera, Human, hFc Tag on ProA-Biosensor can bind Human EGFR, His Tag with an affinity constant of 0.25 µM as determined in BLI assay (Gator® Prime). »
Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.2 - 1.5 ng/mL. »
Immobilized Biotinylated Human EGFR, His-Avi Tag at 1 μg/ml (100 μI/Well) on streptavidin(5 μg/ml) precoated plate. Dose response curve for EGF hFc Chimera, Human, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. »
The purity of EGF hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
EGF hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human EGFR, His Tag captured on CM5 Chip via anti-his antibody can bind EGF hFc Chimera, Human, hFc Tag with an affinity constant of 0.39 nM as determined in SPR assay (Biacore T200). »
| Target Background | The epidermal growth factor (EGF) family of peptides encodes several proteins that can function as growth factors. The EGF-like peptides, with the exception of proteins of the EGF-CFC subfamily, bind and activate tyrosine kinase receptors that belong to the erbB family. |
| Synonyms | HOMG4; EGF; URG |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
