| Species |
Human |
| Protein Construction |
CD48/SLAMF2 (Gln27-Ser220)
Accession # P09326-1 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human 2B4, His Tag at 1μg/ml (100μl/well) on the plate can bind CD48/SLAMF2 hFc Chimera, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
48.9 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 62-72 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. |
| Synonyms |
CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1; mCD48; MEM-102; TCT.1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
