| Species |
Human |
| Protein Construction |
BTN2A1&BTN3A1 complex [Gln29-Ala248 (BTN2A1) acidic tail and Gln30-Gly254 (BTN3A1) basic tail]
Accession # Q7KYR7-1 (BTN2A1) & O00481-1 (BTN3A1)] |
His |
Flag |
| N-term |
|
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized BTN2A1&BTN3A1 complex, His & Flag, Human at 1μg/ml (100μl/well) on the plate can bind AntiBTN3A1 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
30.74 kDa (BTN2A1) and 30.25 kDa (BTN3A1) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 53-63 kDa (BTN2A1) and 33-38 kDa (BTN3A1) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
BTN2A1 and BTN3A1 clearly play highly distinct roles in Vγ9Vδ2 T cell detection of P-Ag-exposed cells. BTN2A1&BTN3A1, a protein complex which contains heterodimeric BTN2A1 and BTN3A1 extracellular domains (ECD), is critical to enhance cytotoxic killing by Vγ9Vδ2 T cells. |
| Synonyms |
BTN3A1&BTN2A1; BTN2A1&BTN3A1; |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
