目录产品 » B7-1/CD80, His & Avi, Human
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B7-1/CD80, His & Avi, Human

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
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Z05053-100

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产品简介
Species Human
Protein Construction
B7-1/CD80 (Val35-Asn242)
Accession # P33681-1		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 26.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
B7-1/CD80, His & Avi, Human

Immobilized Human CTLA-4, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for B7-1/CD80, His & Avi, Human, His Tag with the EC50 of 1.33μg/ml determined by ELISA. »

B7-1/CD80, His & Avi, Human

The purity of  B7-1/CD80, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

B7-1/CD80, His & Avi, Human

B7-1/CD80, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

B7-1/CD80, His & Avi, Human

B7-1/CD80, His & Avi, Human, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Synonyms CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.