| ￥3500 | |
| Z04951-100 | |
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| Species | Cynomolgus/Rhesus macaque | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 31.5 kDa (FCGRT) and 11.6 kDa (B2M) | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT) and 13-15 kDa (B2M) based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized FcRn, His, Cynomolgus/Rhesus macaque, His Tag at 2 μg /ml (100 μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 8.6ng/ml determined by ELISA. »
The purity of FcRn, His, Cynomolgus/Rhesus macaque is greater than 95% as determined by SEC-HPLC. »
FcRn, His, Cynomolgus/Rhesus macaque on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
FcRn, His, Cynomolgus/Rhesus macaque, His Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.18 μM as determined in SPR assay (Biacore T200). »
Cynomolgus Serum Albumin, His Tag immobilized on CM5 Chip can bind FcRn, His, Cynomolgus/Rhesus macaque, His Tag with an affinity constant of 0.13 μM as determined in SPR assay (Biacore T200). »
| Target Background | The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT&B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer. |
| Synonyms | FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
