| Species |
Cynomolgus |
| Protein Construction |
| His |
GDF15 (Arg193-Val308)
Accession # G7PWZ3 |
| N-term |
C-term |
|
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1 EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized GDF15, His, Cynomolgus at 5 μg/ml (100μl/well) on the plate can bind Biotinylated Cynomolgus GFRAL, His Tag. Test result was comparable to standard batch. |
| Expression System |
E.coli |
| Theoretical Molecular Weight |
14.15 kDa |
| Apparent Molecular Weight |
The protein migrates to 15-18 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22 μm filtered solution in 50mM HAc (pH 2.9). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized GDF15, His, Cynomolgus, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 39.9 ng/ml determined by ELISA.
GDF15, His, Cynomolgus on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
| Target Background |
Growth and differentiation factor 15 (GDF15) is an inflammation-associated hormone with poorly defined biology. Here, we investigated the role of GDF15 in bacterial and viral infections. Inflammation induced GDF15, and that GDF15 was necessary for surviving both bacterial and viral infections, as well as sepsis. The protective effects of GDF15 were largely independent of pathogen control or the magnitude of inflammatory response, suggesting a role in disease tolerance. |
| Synonyms |
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; Placental TGF-β; PTGF-β; PTGFBPTGF-β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
