Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Anti-Alkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus, His Tag with an affinity constant of 0.11 μM as determined in SPR assay (Biacore T200).

Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus

Alkaline phosphatase can be considered 'our favorite enzyme' for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.

产品编号	Z04729
规格	100 μg 500 μg 大单询价
数量
价格	￥3500


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产品简介

Species

Cynomolgus

Protein Construction

Alkaline Phosphatase (Placental type) /ALPP (Ile21-Thr503) Accession # XP_045223825.1	His
N-term	C-term

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

AntiAlkaline Phosphatase Antibody captured on CM5 Chip via Protein A can bind Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus in SPR assay (Biacore T200). Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

53.69 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in 25mM Tris, 100mM NaCl, 1mM MgCl₂ (pH 7.5).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

图例

Alkaline Phosphatase (Placental type) /ALPP, His, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.