| Species |
Cynomolgus |
| Protein Construction |
4-1BB Ligand/TNFSF9 (Arg68-Glu251)
Accession # XP_015296398.1 |
hFc |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2μg/ml (100μl/Well) on the plate can bind 4-1BB Ligand/TNFSF9 hFc Chimera, Cynomolgus. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
46 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 50-52 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner. |
| Synonyms |
41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
