| ￥3500 | |
| Z04721-100 | |
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| Species | Cynomolgus | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 46 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-52 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded Cynomolgus 4-1BB Ligand, hFc Tag on ProA-Biosensor can bind 4-1BB Ligand/TNFSF9 hFc Chimera, Cynomolgus, His Tag with an affinity constant of 23.5 nM as determined in BLI assay (Gator® Prime). »
Immobilized 4-1BB Ligand/TNFSF9 hFc Chimera, Cynomolgus, His Tag at 0.5 μg/ml (100 μI/well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA. »
The purity of 4-1BB Ligand/TNFSF9 hFc Chimera, Cynomolgus is greater than 95% as determined by SEC-HPLC. »
4-1BB Ligand/TNFSF9 hFc Chimera, Cynomolgus on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner. |
| Synonyms | 41BB Ligand; 4-1BB Ligand; 4-1BBL; CD137L; TNFSF9 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
