| ￥5800 | |
| Z04585-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 28.1 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized PD-L1/B7-H1[Biotin], His & Avi, Human, His-Avi Tag at 1μg/ml (100μl/Well) on streptavidin(5mg/ml) precoated plate. Dose response curve for Human PD-1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. »
Immobilized Anti-PD-L1 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for PD-L1/B7-H1[Biotin], His & Avi, Human, His Tag with the EC50 of 9.4ng/ml determined by ELISA. »
The purity of PD-L1/B7-H1[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
PD-L1/B7-H1[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
| Synonyms | CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
