| ￥5800 | |
| Z04559-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by Bis-Tris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. mmobilized MICB[Biotin], His & Avi, Human at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human NKG2D, hFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 34.4 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 48-65 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized MICB[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA. »
MICB[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »
Immobilized Anti-MICB Antibody, hFc Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for MICB[Biotin], His & Avi, Human , His Tag with the EC50 of 0.43μg/ml determined by ELISA. »
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind MICB[Biotin], His & Avi, Human, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200). »
| Target Background | MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. |
| Synonyms | MICB; MIC-B; PERB11.2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
