Immobilized Human ITGAV&ITGB6, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for LAP (TGF beta 1)[Biotin], His & Avi, Human, His Tag with the EC50 of 0.11 μg/ml determined by ELISA.

LAP (TGF beta 1)[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

LAP (TGF beta 1)[Biotin], His & Avi, Human

Latent TGF beta 1 cDNA encodes a 390 amino acid precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1. Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex.

产品编号	Z04532
规格	100 μg 500 μg 大单询价
数量
价格	￥6600


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产品简介

Species

Human

Protein Construction

Avi	His	LAP (TGF beta 1) [Leu30-Arg278 (C33S)] Accession # P01137
N-term		C-term

Conjugate

Biotin

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Human ITGAV&ITGB6, His Tag at 2μg/ml (100μl/well) on the plate can bind LAP (TGF beta 1)[Biotin], His & Avi, Human. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

31.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

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