| ￥7400 | |
| Z04510-100 | |
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| Species | Human&Mouse | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml) can bind Human IL23R, mFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 21.7 kDa (Human IL-23 alpha) & 35.8 kDa (Mouse IL-12 beta) | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 22-25 kDa (Human IL-23 alpha) & 45-60 kDa (Mouse IL-12 beta) based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-23R, mFc Tag with the EC50 of 37.1 ng/ml determined by ELISA. »
The purity of IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse is greater than 95% as determined by SEC-HPLC. »
IL-23 alpha&IL-12 beta[Biotin], His & Avi, Human&Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Interleukin (IL)-12 and IL-23 belong to the IL-12 type family and are composite cytokines, consisting of the common β subunit p40 and the specific cytokine α subunit p35 and p19, respectively. IL-12 signals via the IL-12Rβ1·IL-12Rβ2 receptor complex, and IL-23 uses also IL-12Rβ1 but engages IL-23R as second receptor. |
| Synonyms | IL23 alpha; IL12 beta; IL23 alpha&IL12 beta ; IL12 β; IL23 alpha&IL12 β |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
