| Species |
Human |
| Protein Construction |
IL-22R alpha 1 & IL-10R beta [His16-Thr228 (IL-22R alpha 1) and Met20-Ser220 (IL-10R beta)]
Accession # Q8N6P7 (IL-22R alpha 1) & Q08334 (IL-10R beta) |
hFc |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human IL22, His Tag at 5μg/ml (100μl/well) on the plate can bind IL-22R alpha 1&IL-10R beta hFc Chimera[Biotin], Avi, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
52.07 kDa (IL-22R alpha 1) and 48.85 kDa (IL-10R beta) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
IL-22Rα1&IL-10Rβ are receptors for IL-22 and IL-10, mediate the activation of JAK-STAT, MAPK and other signaling pathways, and participate in the process of many inflammatory diseases. IL-22 Rα1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL10 Rβ or IL20 Rβ and the respective ligands is required for signal transduction[1]. |
| Synonyms |
IL-22R alpha 1; IL-10R beta; IL-22Rα1&IL-10Rβ |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
