Species | Human | ||||
Protein Construction |
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Conjugate | Biotin | ||||
Purity | > 95% as determined by Bis-Tris PAGE | ||||
Endotoxin Level | Less than 1 EU per ug by the LAL method. | ||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized IL-15RA&IL-15 [Biotin], His, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate (5 μg/ml) can bind Human IL2 R beta, hFc Tag. Test result was comparable to standard batch. | ||||
Expression System | HEK293 | ||||
Theoretical Molecular Weight | 23.8 kDa | ||||
Apparent Molecular Weight | Due to glycosylation, the protein migrates to 25-28 kDa&30-35 kDa based on Bis-Tris PAGE result. | ||||
Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized IL-15RA&IL-15 [Biotin], His, Human, His Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-2 R beta, hFc Tag with the EC50 of 7.5 ng/ml determined by ELISA. »
IL-15RA&IL-15 [Biotin], His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Target Background | Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development. |
Synonyms | IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.