目录产品 » IL-13[Biotin], His & Avi, Human
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IL-13[Biotin], His & Avi, Human

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
￥5800
Z04492-100

产品简介
Species Human
Protein Construction
Avi His IL-13 (Gly21-Asn132)
Accession # AAK53823
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-13[Biotin], His & Avi, Human at 5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind Human IL­13Ra2, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 15.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 15 kDa and 35-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
IL-13[Biotin], His & Avi, Human

Immobilized IL-13[Biotin], His & Avi, Human, His Avi Tag at 5 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 0.81 μg/ml determined by ELISA. »

IL-13[Biotin], His & Avi, Human

The purity of IL-13[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

IL-13[Biotin], His & Avi, Human

IL-13[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »

IL-13[Biotin], His & Avi, Human

Human IL-13Ra1, hFc Tag captured on CM5 Chip via Protein A can bind IL-13[Biotin], His & Avi, Human, His Tag with an affinity constant of 25.59 nM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Synonyms IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.