| ￥8200 | |
| Z04491-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized AntiIL12 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate can bind IL-12[Biotin], His & Avi, Human. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 34.7 kDa (IL-12B) and 25.5 kDa (IL-12A) | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Anti-IL-12 Antibody, hFc Tag at 1 μg/ml(100 μl/well) on the plate. Dose response curve for IL-12[Biotin], His & Avi, Human, His Tag with the EC50 of 7.5 ng/ml determined by ELISA. »
The purity of IL-12[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
IL-12[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably. |
| Synonyms | CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
