| ￥5800 | |
| Z04475-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized AntiGMCSF R alpha Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate can bind GM-CSF R alpha[Biotin], His & Avi, Human. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 37.7 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Anti-GM-CSF R alpha Antibody, hFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for GM-CSF R alpha[Biotin], His & Avi, Human, His Tag with the EC50 of 6.8 ng/ml determined by ELISA. »
The purity of GM-CSF R alpha[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
GM-CSF R alpha[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »
GM-CSF R alpha[Biotin], His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human GM-CSF, No Tag with an affinity constant of 1.68 nM as determined in SPR assay (Biacore T200). »
| Target Background | In the first trimester, the GM-CSF-R was found to be present on villous cytotrophoblast and all populations of extravillous trophoblast. Expression by villous syncytiotrophoblast was weak or absent, but this increased markedly by term. GM-CSF-R were also expressed by fetal Hofbauer cells within the mesenchyme of the chorionic villi and by uterine glandular epithelium and decidual macrophages within maternal decidua. |
| Synonyms | GM-CSF-R-alpha; GMCSFR-alpha; GMR-alpha; CSF2R; CSF2RY; CSF2RA; CDw116; CD116; CSF2RAX; CSF2RAY; CSF2RX; GMCSFR; GMR; SMDP4 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
