目录产品 » GFRAL/GFR alpha-like[Biotin], His & Avi, Human
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GFRAL/GFR alpha-like[Biotin], His & Avi, Human

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
￥5800
Z04471-100

产品简介
Species Human
Protein Construction
GFRAL/GFR alpha-like (Ser19-Glu351)
Accession # Q6UXV0		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human GDF15, His Tag at 5μg/ml (100μl/well) on the plate can bind GFRAL/GFR alpha-like[Biotin], His & Avi, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 40.7 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-62 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
GFRAL/GFR Alpha-like[Biotin], His & Avi, Human

Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : GFRAL/GFR alpha-like[Biotin], His & Avi, Human, His Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 40.8 ng/ml. »

GFRAL/GFR Alpha-like[Biotin], His & Avi, Human

Immobilized Human GDF15, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for GFRAL/GFR alpha-like[Biotin], His & Avi, Human, His-Avi Tag with the EC50 of 84.2 ng/ml determined by ELISA. »

GFRAL/GFR Alpha-like[Biotin], His & Avi, Human

The purity of GFRAL/GFR alpha-like[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

GFRAL/GFR Alpha-like[Biotin], His & Avi, Human

GFRAL/GFR alpha-like[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »

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技术背景
Target Background GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Synonyms GFR alpha-like; GFRAL; GRAL; C6orf144
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.