| ￥5800 | |
| Z04451-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate can bind Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 24.7 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized Anti-Fc gamma RIIIA Antibody, hFc Tag at 5 μg/ml (100 μL/well) on the plate. Dose response curve for Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human, His Tag with the EC50 of 8.2 ng/ml determined by ELISA. »
The purity of Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIIA/CD16a (V176)[Biotin], His & Avi, Human , His-Avi Tag with an affinity constant of 0.539 μM as determined in SPR assay (Biacore T200). »
| Target Background | The Human Fc gamma RIIIA/CD16a Protein is a receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. |
| Synonyms | Fc gamma RIIIA; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; CD16A; FCG3; IGFR3; FcRIIIa; FcRIII; CD16; IMD20 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
