目录产品 » Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human
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Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
￥5800 在线订购包邮(港澳台除外)
Z04450-100

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产品简介
Species Human
Protein Construction
Fc gamma RIIA/CD32a (R167)[Gln34-Ile218(R167)]
Accession # P12318-1		 His Avi
N-term C-term
Conjugate Biotin
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Fc gamma RIIA/CD32a (R167) [Biotin], His & Avi, Human on CM5 Chip via anti­-his antibody can bind Rituximab in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 23.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
Fc Gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human

The purity of Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Fc Gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human

Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

Fc Gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human

Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human , His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.17 μM as determined in SPR assay (Biacore T200) (QC Test). »

Fc Gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human

Rituximab captured on CM5 Chip via Protein A can bind Fc gamma RIIA/CD32a (R167)[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 1.03 μM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Synonyms Fc gamma RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; FCG2; CD32A; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.