| ￥5800 | |
| Z04442-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity |
> 95% as determined by BisTris PAGE > 95% as determined by SECHPLC |
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| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized DNAM-1/CD226[Biotin], His & Avi, Human at 1μg/ml (100μl/well) on the streptavidin precoated plate(5μg/ml) can bind Human CD155, mFc Tag. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 28.9 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized DNAM-1/CD226[Biotin], His & Avi, Human, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Human CD155, mFc Tag with the EC50 of 8.6 ng/ml determined by ELISA. »
The purity of DNAM-1/CD226[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
DNAM-1/CD226[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind DNAM-1/CD226[Biotin], His & Avi, Human, His-Avi Tag with an affinity constant of 264nM as determined in SPR assay (Biacore T200). »
| Target Background | DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2. |
| Synonyms | CD226 molecule; CD226; DNAM1; PTA1; TLiSA1 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
