| Species |
Human |
| Protein Construction |
DKK1 C terminal Domain (Met178-His266)
Accession # O94907 |
hFc |
Avi |
| N-term |
|
C-term |
|
| Conjugate |
Biotin |
| Purity |
> 95% as determined by Bis-Tris PAGE |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Human LRP6, mFc Tag at 5μg/ml (100μl/well) on the plate can bind DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
38.69 kDa |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
![DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human](/product/documents/down?doc_name=Z04438 ELISA.jpg&file=scm_files/productFile_notes/2025/05/13/Z04438 ELISA.jpg "DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human")
Immobilized Human LRP-6, mFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human, hFc Tag with the EC50 of 2.27 μg/ml determined by ELISA.
![DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human](/product/documents/down?doc_name=Z04438 PAGE.png&file=scm_files/productFile_notes/2025/05/13/Z04438 PAGE.png "DKK1 C Terminal Domain HFc Chimera[Biotin], Avi, Human")
DKK1 C terminal Domain hFc Chimera[Biotin], Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
| Target Background |
Dickkopf-1 (Dkk1), the founding and best-studied member of the Dkk family, functions as an antagonist of canonical Wnt/β-catenin. Dkk1 is considered to play a broad role in a variety of biological processes. |
| Synonyms |
Dickkopf-related 1; Dickkopf-1; Dkk-1; Dkk1; hDkk-1; hDkk1; SK |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
