Species | Human | ||||||
Protein Construction |
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Conjugate | Biotin | ||||||
Purity | > 95% as determined by Bis-Tris PAGE | ||||||
Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized CCL5[Biotin], His & Avi, Human at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate can bind AntiCCL5 Antibody, hFc Tag. Test result was comparable to standard batch. | ||||||
Expression System | E.coli | ||||||
Theoretical Molecular Weight | 10.89 kDa | ||||||
Apparent Molecular Weight | The protein has a predicted MW of 10.89 kDa same as Bis-Tris PAGE result. | ||||||
Formulation | Lyophilized from 0.22μm filtered solution in 4mM HCI. | ||||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4mM HCI. | ||||||
Storage & Stability | Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
Immobilized CCL5[Biotin], His & Avi, Human, His Avi Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate(5 μg/ml). Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 5.2 ng/ml determined by ELISA. »
CCL5[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Target Background | The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers. |
Synonyms | MuRantes; SIS-delta; Scya5; Ccl5; D17S136E; eoCP; RANTES; SCYA5; SISd; TCP228; CCL5 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.